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Directed evolution of the quorum-sensing regulator EsaR for increased signal sensitivity.
Shong J, Huang YM, Bystroff C, Collins CH
ACS Chem Biol. 2013 Apr 19;8(4):789-95. doi: 10.1021/cb3006402. Epub 2013 Feb 6.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
47640pCS-PesaRluxwt esaR promoter in pCS26
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47641pCS-PluxIluxwt luxI promoter in pCS26
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47643pAC-EsaRwt esaR gene under control of Plac in pACYC184
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47644pAC-EsaR-I70V/D91GpAC-EsaR with a208g and a272g mutations in esaR
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47645pAC-EsaR-V220ApAC-EsaR with a c659t mutation in esaR
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47646pAC-EsaR-D91GpAC-EsaR with a a272g mutation in esaR
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47647pAC-EsaR-I70V/D91G/V220ApAC-EsaR with a208g, a272g, c659t mutations in esaR
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47648pAC-EsaR-D91G/V220ApAC-EsaR with a272g and c659t mutations in esaR
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47663pAC-EsaR-I70VpAC-EsaR with a208g mutation in esaR
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