Directed evolution of the quorum-sensing regulator EsaR for increased signal sensitivity.
Shong J, Huang YM, Bystroff C, Collins CH
ACS Chem Biol. 2013 Apr 19;8(4):789-95. doi: 10.1021/cb3006402. Epub 2013 Feb 6. PubMed Article
Shong J, Huang YM, Bystroff C, Collins CH
ACS Chem Biol. 2013 Apr 19;8(4):789-95. doi: 10.1021/cb3006402. Epub 2013 Feb 6. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 47640 | pCS-PesaRlux | wt esaR promoter in pCS26 |
| 47641 | pCS-PluxIlux | wt luxI promoter in pCS26 |
| 47643 | pAC-EsaR | wt esaR gene under control of Plac in pACYC184 |
| 47644 | pAC-EsaR-I70V/D91G | pAC-EsaR with a208g and a272g mutations in esaR |
| 47645 | pAC-EsaR-V220A | pAC-EsaR with a c659t mutation in esaR |
| 47646 | pAC-EsaR-D91G | pAC-EsaR with a a272g mutation in esaR |
| 47647 | pAC-EsaR-I70V/D91G/V220A | pAC-EsaR with a208g, a272g, c659t mutations in esaR |
| 47648 | pAC-EsaR-D91G/V220A | pAC-EsaR with a272g and c659t mutations in esaR |
| 47663 | pAC-EsaR-I70V | pAC-EsaR with a208g mutation in esaR |