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pSL690
(Plasmid #47753)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 47753 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL1 Blue
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    dCas9-VP64
  • Alt name
    inactive Cas9 nuclease
  • Alt name
    VP64 activation domain
  • Species
    Synthetic; Streptococcus pyogenes
  • Mutation
    D10A and H840A mutations in Cas9
  • Promoter CMV
  • Tags / Fusion Proteins
    • NLS (C terminal on insert)
    • 3x FLAG (C terminal on insert)
    • VP64 (C terminal on insert)

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    dCas9 amplified from Jennifer Doudna lab--plasmid pMJ841 (Addgene plasmid # 39318)
  • Terms and Licenses

Depositor Comments

Plasmid Features:
Cas9 = 409-4512
NLS = 4519-4539
3X FLAG = 4546-4611
VP64 = 4633-4794

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSL690 was a gift from Keith Joung (Addgene plasmid # 47753 ; http://n2t.net/addgene:47753 ; RRID:Addgene_47753)
  • For your References section:

    CRISPR RNA-guided activation of endogenous human genes. Maeder ML, Linder SJ, Cascio VM, Fu Y, Ho QH, Joung JK. Nat Methods. 2013 Jul 25. doi: 10.1038/nmeth.2598. 10.1038/nmeth.2598 PubMed 23892898