pLew90β-GW/T7/PARP SAS/luciferase/Aldolase 3′UTR
(Plasmid
#48157)
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PurposeTransgenically expresses firefly luciferase protein in T. cruzi
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Depositing Lab
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Depositing OrganizationUniversity of Georgia
Located in United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 48157 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 * | |
| DNA | 48157-D50 | 50 μg of DNA in Tris buffer | $495 * | ||
| DNA | 48157-D100 | 100 μg of DNA in Tris buffer | $585 * | ||
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Backbone
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Vector backbonepLew90β-T7-GW-Neo
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Backbone manufacturerCourtney Boehlke
- Backbone size w/o insert (bp) 7000
- Total vector size (bp) 8000
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Modifications to backbonesee paper
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Vector typeLuciferase ; T. cruzi expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameluciferase
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Alt namefirefly luciferase
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SpeciesP. pyralis
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Insert Size (bp)1640
- Promoter T7
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer TAATACGACTCACTATAGGG
- 3′ sequencing primer GCTAGTTATTGCTCAGCGG
- (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made bypPT7:Otet/Luc vector (a gift from John E. Donelson, University of Iowa, Iowa City, IA)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 48157-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
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Industry Terms
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 48157-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLew90β-GW/T7/PARP SAS/luciferase/Aldolase 3′UTR was a gift from Rick Tarleton (Addgene plasmid # 48157 ; http://n2t.net/addgene:48157 ; RRID:Addgene_48157) -
For your References section:
In vitro and in vivo high-throughput assays for the testing of anti-Trypanosoma cruzi compounds. Canavaci AM, Bustamante JM, Padilla AM, Perez Brandan CM, Simpson LJ, Xu D, Boehlke CL, Tarleton RL. PLoS Negl Trop Dis. 2010 Jul 13;4(7):e740. doi: 10.1371/journal.pntd.0000740. 10.1371/journal.pntd.0000740 PubMed 20644616
Map uploaded by the depositor.
