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pFA6a-ura4MX6
(Plasmid #49184)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 49184 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry

Depositor Comments

Note that there are two adenines in Addgene's T7 quality control sequence that introduce a HindIII site and an AflII site into the plasmid. Note that these discrepancies with the depositor sequence do NOT affect the function of the plasmid. The vector is used as a template for PCR to amplify DNA cassettes to modify chromosomal gene loci. The additional adenines are not within the primer sequences to amplify DNA cassettes for gene manipulation.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFA6a-ura4MX6 was a gift from Eishi Noguchi (Addgene plasmid # 49184 ; http://n2t.net/addgene:49184 ; RRID:Addgene_49184)
  • For your References section:

    New vectors for epitope tagging and gene disruption in Schizosaccharomyces pombe. Gadaleta MC, Iwasaki O, Noguchi C, Noma K, Noguchi E. Biotechniques. 2013 Nov;55(5):257-63. doi: 10.2144/000114100. 10.2144/000114100 PubMed 24215641