Skip to main content
Addgene
Open Menu

pDEST-mCherry-GJA1-20k-N1
(Plasmid #49861)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 49861 Standard format: Plasmid sent in bacteria as agar stab 1 $94
Add to Cart
DNA 49861-D50 50 μg of DNA in Tris buffer $495
Add to Cart
DNA 49861-D100 100 μg of DNA in Tris buffer $585
Add to Cart

Backbone

  • Vector backbone
    pmcherry-N1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4722
  • Total vector size (bp) 5232
  • Modifications to backbone
    Gateway reading frame cassette c.1 inserted between Afe I and Sma I sites of MCS.
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GJA1-20k
  • Alt name
    GJA1
  • Alt name
    Connexin 43
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    510
  • Mutation
    GJA1-20k corresponds to residues 213 to 382 to Connexin 43. No stop codon to create c-terminal fusion
  • GenBank ID
    NM_000165.3
  • Entrez Gene
    GJA1 (a.k.a. AVSD3, CMDR, CX43, EKVP, EKVP3, GJAL, HLHS1, HSS, ODDD, PPKCA)
  • Promoter CMV
  • Tag / Fusion Protein
    • mCherry (C terminal on backbone)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDEST-mCherry-GJA1-20k-N1 was a gift from Robin Shaw (Addgene plasmid # 49861 ; http://n2t.net/addgene:49861 ; RRID:Addgene_49861)

  • For your References section:

    Autoregulation of Connexin43 Gap Junction Formation by Internally Translated Isoforms. Smyth JW, Shaw RM. Cell Rep. 2013 Nov 5. pii: S2211-1247(13)00578-0. doi: 10.1016/j.celrep.2013.10.009. 10.1016/j.celrep.2013.10.009 PubMed 24210816
Related items:
Commonly requested with: