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Autoregulation of Connexin43 Gap Junction Formation by Internally Translated Isoforms.
Smyth JW, Shaw RM
Cell Rep. 2013 Nov 5. pii: S2211-1247(13)00578-0. doi: 10.1016/j.celrep.2013.10.009.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
49851pcDNA3.2-Cx43-HAEncodes human connexin 43 with a C-terminal HA epitope fusion tag
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49852pcDNA3.2-Cx43-siResistEncodes human connexin 43 with wobble mutations conferring resistance against an siRNA
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49853pcDNA3.2-Cx43-NoStartEncodes human connexin 43 with the start methionine codon mutated to isoleucine and with wobble mutations conferring resistance against an siRNA
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49854pcDNA3.2-Cx43-M100L-siResistEncodes human connexin 43 with M100 mutated to L and wobble mutations conferring resistance against an siRNA
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49855pcDNA3.2-Cx43-M100/125L-siResistEncodes human connexin 43 with M100 and M125 mutated to L and wobble mutations conferring resistance against an siRNA
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49856pcDNA3.2-Cx43-M100/125/147L-siResistEncodes human connexin 43 with M100, M125, and M147 mutated to L and wobble mutations conferring resistance against an siRNA
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49857pcDNA3.2-Cx43-ML-HAEncodes human connexin 43 with all internal methionines mutated to leucine and a C_terminal HA epitope fusion tag
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49858pcDNA3.2-Cx43-M100/125/147/213-siResistEncodes human connexin 43 with M100, M125, M147, and M213 mutated to L and wobble mutations conferring resistance against an siRNA
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49859pcDNA3.2-GJA1-20K-V5Encodes C-terminal fragment of human connexin 43 initiating at M213, with M281 and M320 mutated to L, and a V5 C-terminal fusion tag
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49860pDEST-EGFP-Cx43ML-N1Encodes human connexin 43 with all internal methionines mutated to leucine and a C_terminal EGFP fusion tag
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49861pDEST-mCherry-GJA1-20k-N1Encodes C-terminal fragment of human connexin 43 initiating at M213 with a V5 C-terminal fusion tag
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