|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||50360||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backboneUnknown (a derivative of pVP22/Myc-His)
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
Alt nameargonaute RISC catalytic component 1
Alt nameargonaute 1
SpeciesH. sapiens (human)
Entrez GeneAGO1 (a.k.a. EIF2C, EIF2C1, GERP95, Q99, hAgo1)
- Promoter CMV
/ Fusion Protein
- myc (C terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pcDNAM-human eIF2C1-WT-Myc was a gift from Kumiko Ui-Tei (Addgene plasmid # 50360 ; http://n2t.net/addgene:50360 ; RRID:Addgene_50360)
For your References section:Short-interfering-RNA-mediated gene silencing in mammalian cells requires Dicer and eIF2C translation initiation factors. Doi N, Zenno S, Ueda R, Ohki-Hamazaki H, Ui-Tei K, Saigo K. Curr Biol. 2003 Jan 8;13(1):41-6. 10.1016/S0960-9822(02)01394-5 PubMed 12526743
Map uploaded by the depositor.