Purpose(Empty Backbone) Backbone: pRBS-01, Origin Of Replication: p15a, Selection Markers: Amp, Promoters: PLacUV5,
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||50597||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector typeBacterial Expression, Synthetic Biology ; Empty Vector
Growth in Bacteria
- Promoter PLacUV5
- Cloning method Unknown
- 5′ sequencing primer lac promoter
- 3′ sequencing primer NA (Common Sequencing Primers)
Terms and Licenses
Note that Addgene was not able to confirm some key features of this plasmid without a reference sequence.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pY1 was a gift from Jay Keasling (Addgene plasmid # 50597 ; http://n2t.net/addgene:50597 ; RRID:Addgene_50597)
For your References section:Modular engineering of L-tyrosine production in Escherichia coli. Juminaga D, Baidoo EE, Redding-Johanson AM, Batth TS, Burd H, Mukhopadhyay A, Petzold CJ, Keasling JD. Appl Environ Microbiol. 2012 Jan;78(1):89-98. doi: 10.1128/AEM.06017-11. Epub 2011 Oct 21. 10.1128/AEM.06017-11 PubMed 22020510