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pOTTC292 - pAAV EF1a floxed hChR2(H134R)-EYFP
(Plasmid #50834)


Item Catalog # Description Quantity Price (USD)
Plasmid 50834 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
    Addgene 20298
  • Backbone manufacturer
    Karl Deisseroth
  • Backbone size w/o insert (bp) 5500
  • Total vector size (bp) 7266
  • Modifications to backbone
    Removed double floxed inverted open reading frame (hChR2-EYFP) using KpnI and EcoRI digestion. Used In-Fusion cloning to recombine backbone with PCR-based insert (KpnI-loxP-hChR2-EYFP-loxP-EcoRI).
  • Vector type
    Mammalian Expression, AAV, Cre/Lox

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
    hChR2 (H134R)
  • Alt name
    Channel Rhodopsin 2
  • Species
    C. reinhardtii
  • Mutation
  • GenBank ID
  • Promoter EF1a
  • Tag / Fusion Protein
    • EYFP (C terminal on insert)

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer EF1a_F1
  • 3′ sequencing primer WPRE_R1
  • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    The ORF for hChR2-EYFP, and the AAV vector backbone was obtained from Karl Deisseroth, but is also available from Addgene (Plasmid 20298).

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pOTTC292 - pAAV EF1a floxed hChR2(H134R)-EYFP was a gift from Brandon Harvey (Addgene plasmid # 50834 ; ; RRID:Addgene_50834)
  • For your References section:

    Serotonergic versus nonserotonergic dorsal raphe projection neurons: differential participation in reward circuitry. McDevitt RA, Tiran-Cappello A, Shen H, Balderas I, Britt JP, Marino RAM, Chung SL, Richie CT, Harvey BK, Bonci A. Cell Rep. 2014 Sep 25;8(6):1857-1869. doi: 10.1016/j.celrep.2014.08.037. Epub 2014 Sep 18. 10.1016/j.celrep.2014.08.037 PubMed 25242321