pEF5/FRT/DEST-3xHA/mGli3/Flag P5-6A
(Plasmid
#51249)
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PurposeEncodes N-3xHA-TEV-mouseGli3-Flag-C; amino acids 980,1006 mutated to Ala
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Depositing Lab
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Depositing OrganizationStanford University
Located in United States of America -
Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 51249 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 51249-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 51249-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepEF5-FRT-DEST
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Backbone manufacturerInvitrogen
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Vector typeMammalian Expression ; Flpin system
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameGli3
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SpeciesM. musculus (mouse)
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Mutationamino acids 980,1006 mutated to Ala
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GenBank ID14634
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Entrez GeneGli3 (a.k.a. Bph, GLI3-190, GLI3FL, Pdn, Xt, add)
- Promoter EF1a
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Tags
/ Fusion Proteins
- 3xHA-TEV (N terminal on insert)
- Flag (C terminal on insert)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer T7
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 51249-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 51249-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEF5/FRT/DEST-3xHA/mGli3/Flag P5-6A was a gift from Rajat Rohatgi (Addgene plasmid # 51249 ; http://n2t.net/addgene:51249 ; RRID:Addgene_51249) -
For your References section:
Gli Protein Activity Is Controlled by Multisite Phosphorylation in Vertebrate Hedgehog Signaling. Niewiadomski P, Kong JH, Ahrends R, Ma Y, Humke EW, Khan S, Teruel MN, Novitch BG, Rohatgi R. Cell Rep. 2013 Dec 24. pii: S2211-1247(13)00733-X. doi: 10.1016/j.celrep.2013.12.003. 10.1016/j.celrep.2013.12.003 PubMed 24373970