pBAD24-sfGFPx1-SD2
(Plasmid
#51561)
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PurposeSuperfolder GFP ORF cloned into pBAD24 for expression in E. coli (variant with increased in vivo fluorescence).
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Depositing Labs
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 51561 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepBAD24
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsMaintained in DH5alpha, assayed in MG1655 after retransformation.
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namesuperfolder GFP
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SpeciesSynthetic
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MutationSynonymous mutation on Val22 (GTC to GTT)
Resource Information
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A portion of this plasmid was derived from a plasmid made byMutant from pBAD24-sfGFPx1
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBAD24-sfGFPx1-SD2 was a gift from Sankar Adhya & Francisco Malagon (Addgene plasmid # 51561) -
For your References section:
RNase III is required for localization to the nucleoid of the 5' pre-rRNA leader and for optimal induction of rRNA synthesis in E. coli. Malagon F. RNA. 2013 Sep;19(9):1200-7. doi: 10.1261/rna.038588.113. Epub 2013 Jul 26. 10.1261/rna.038588.113 PubMed 23893733