p413TEF-SirT1
(Plasmid
#51746)
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Purposeoverexpression of human SirT1 in S. cerevisiae, HIS3 marker
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 51746 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonep413TEF
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Vector typeYeast Expression
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Selectable markersHIS3
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSirT1
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Alt nameSIRT1 sirtuin 1
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SpeciesH. sapiens (human)
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Insert Size (bp)2244
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GenBank IDNM_012238.4
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Entrez GeneSIRT1 (a.k.a. SIR2, SIR2L1, SIR2alpha)
- Promoter TEF1
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site SalI (destroyed during cloning)
- 5′ sequencing primer Tef1 (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
p413TEF-SirT1 was a gift from Markus Ralser (Addgene plasmid # 51746 ; http://n2t.net/addgene:51746 ; RRID:Addgene_51746) -
For your References section:
Interfering with glycolysis causes Sir2-dependent hyper-recombination of Saccharomyces cerevisiae plasmids. Ralser M, Zeidler U, Lehrach H. PLoS One. 2009;4(4):e5376. doi: 10.1371/journal.pone.0005376. Epub 2009 Apr 24. 10.1371/journal.pone.0005376 PubMed 19390637
Map uploaded by the depositor.
