Purposeexpression of Xl-VSP2 in Xenopus oocytes or mammalian cells
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||51885||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector typeMammalian Expression ; Xenopus oocyte expression
Growth in Bacteria
Copy numberHigh Copy
Alt namevoltage-sensitive phosphatase 2
SpeciesX. laevis (frog)
- Promoter CMV
- Cloning method Restriction Enzyme
- 5′ cloning site KpnI (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGH Reverse (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
Plasmid encoding Xl-VSP2 (CF520610, clone # 7008398) was purchased from Open Biosystems (Huntsville, AL).
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pcDNA3-Xl-VSP2 was a gift from Laurinda Jaffe (Addgene plasmid # 51885 ; http://n2t.net/addgene:51885 ; RRID:Addgene_51885)
For your References section:Voltage sensitive phosphoinositide phosphatases of Xenopus: their tissue distribution and voltage dependence. Ratzan WJ, Evsikov AV, Okamura Y, Jaffe LA. J Cell Physiol. 2011 Nov;226(11):2740-6. doi: 10.1002/jcp.22854. 10.1002/jcp.22854 PubMed 21618529