Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

CT-Nano-lantern(ATP1)/pENTR1A
(Plasmid #51991)

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 51991 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pENTR1A
  • Backbone manufacturer
    Invitrogen
  • Vector type
    entry vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Nano-lantern_ATP1
  • Species
    Synthetic
  • Insert Size (bp)
    2200
  • Mutation
    S257G in Rluc8
  • Tag / Fusion Protein
    • chloroplast transit peptid (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SalI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer pENTR-F
  • 3′ sequencing primer pENTR-R
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

GenBank accession number: JN859502. Although they differ by only three N-terminal amino acidsin the RLuc8-S257G moiety, their dynamic range and brightness were vastly different. Nano-lantern (ATP1) exhibited enough dynamic range (200%) for functional imaging, and was 1.5-fold brighter than Nano-lantern (ATP0) at ATP-saturating condition. They had similar affinities for ATP.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CT-Nano-lantern(ATP1)/pENTR1A was a gift from Takeharu Nagai (Addgene plasmid # 51991 ; http://n2t.net/addgene:51991 ; RRID:Addgene_51991)
  • For your References section:

    Luminescent proteins for high-speed single-cell and whole-body imaging. Saito K, Chang YF, Horikawa K, Hatsugai N, Higuchi Y, Hashida M, Yoshida Y, Matsuda T, Arai Y, Nagai T. Nat Commun. 2012;3:1262. doi: 10.1038/ncomms2248. 10.1038/ncomms2248 PubMed 23232392