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pLenti-OC-IRES-BSD
(Plasmid #53118)

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 53118 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pLenti-CMV-BSD
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    OCT1
  • Alt name
    POU class 2 homeobox 1
  • Alt name
    POU2F1
  • Species
    H. sapiens (human)
  • Entrez Gene
    POU2F1 (a.k.a. OCT1, OTF1, Oct1Z, oct-1B)
  • Promoter CMV
  • Tag / Fusion Protein
    • P2A (C terminal on insert)

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    Cas9
  • Species
    Streptococcus pyogenes
  • Mutation
    human codon-optimized
  • Tags / Fusion Proteins
    • NLS (N terminal on insert)
    • NLS (C terminal on insert)
    • IRES-BSD (C terminal on insert)

Cloning Information for Gene/Insert 2

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLenti-OC-IRES-BSD was a gift from Wensheng Wei (Addgene plasmid # 53118 ; http://n2t.net/addgene:53118 ; RRID:Addgene_53118)
  • For your References section:

    High-throughput screening of a CRISPR/Cas9 library for functional genomics in human cells. Zhou Y, Zhu S, Cai C, Yuan P, Li C, Huang Y, Wei W. Nature. 2014 Apr 9. doi: 10.1038/nature13166. 10.1038/nature13166 PubMed 24717434