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pLenti-sgRNA-Lib
(Plasmid #53121)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 53121 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pLL3.7, Addgene plasmid #11795
  • Backbone manufacturer
    Luk Parijs Lab
  • Modifications to backbone
    This was constructed by substituting its original U6 promoter in pLL3.7 for the human U6 promoter, ccdB cassette and sgRNA scaffold.
  • Vector type
    Mammalian Expression, Lentiviral, CRISPR
  • Promoter U6

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    ccdB Survival
  • Copy number
    High Copy

Cloning Information

Resource Information

Depositor Comments

A CMV-EGFP reporter cassette is included in the vector to monitor expression.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLenti-sgRNA-Lib was a gift from Wensheng Wei (Addgene plasmid # 53121 ; http://n2t.net/addgene:53121 ; RRID:Addgene_53121)
  • For your References section:

    High-throughput screening of a CRISPR/Cas9 library for functional genomics in human cells. Zhou Y, Zhu S, Cai C, Yuan P, Li C, Huang Y, Wei W. Nature. 2014 Apr 9. doi: 10.1038/nature13166. 10.1038/nature13166 PubMed 24717434