Purpose(Empty Backbone) sgRNA expression construction in a 3rd generation lentiviral backbone
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||53121||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonepLL3.7, Addgene plasmid #11795
Backbone manufacturerLuk Parijs Lab
Modifications to backboneThis was constructed by substituting its original U6 promoter in pLL3.7 for the human U6 promoter, ccdB cassette and sgRNA scaffold.
Vector typeMammalian Expression, Lentiviral, CRISPR
- Promoter U6
Growth in Bacteria
Bacterial Resistance(s)Chloramphenicol and Ampicillin
Growth Strain(s)ccdB Survival
Copy numberHigh Copy
- Cloning method Gateway Cloning
- 5′ sequencing primer hU6-F (Common Sequencing Primers)
A CMV-EGFP reporter cassette is included in the vector to monitor expression.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pLenti-sgRNA-Lib was a gift from Wensheng Wei (Addgene plasmid # 53121 ; http://n2t.net/addgene:53121 ; RRID:Addgene_53121)
For your References section:High-throughput screening of a CRISPR/Cas9 library for functional genomics in human cells. Zhou Y, Zhu S, Cai C, Yuan P, Li C, Huang Y, Wei W. Nature. 2014 Apr 9. doi: 10.1038/nature13166. 10.1038/nature13166 PubMed 24717434