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pMyc3ElbLuc
(Plasmid #53245)

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Full plasmid sequence is not available for this item.

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Item Catalog # Description Quantity Price (USD)
Plasmid 53245 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pG5ElbLuc
  • Modifications to backbone
    The plasmid pG5ElbLuc was prepared from pG5E1bCAT (23) by replacing the chloramphenicol acetyltransferase gene (EcoRI and Sty I fragment) with the firefly luciferase gene (from pGEM-Luc) as a 1745-bp HindIII-StuI blunt-end fragment. The GAL4 binding sites were removed from pG5ElbLuc as a Pst I and Kpn I fragment and replaced with a double-stranded 20-mer oligonucleotide that contains the Elb promoter TATA element (5'-GAGGGTATATAATGTGGTAC- 3' and 5'-CACATTATATACCCTCTGCA-3') to create the plasmid pMycOElbLuc.

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    pMyc3ElbLuc
  • Species
    H. sapiens (human)
  • Mutation
    To prepare plasmids with one, two, three, and four Myc binding sites (underlined), a 26-mer double-stranded oligonucleotide (5'-AGCTTAACTGACCACGTGGTCAACTA- 3' and 5'-AGCTTAGTTGACCACGTGGTCAGTTA- 3') was employed in a ligation reaction to obtain the plasmids pMyc3ElbLuc,
  • Entrez Gene
    MYC (a.k.a. MRTL, MYCC, bHLHe39, c-Myc)

Resource Information

  • Terms and Licenses
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMyc3ElbLuc was a gift from Roger Davis (Addgene plasmid # 53245)
  • For your References section:

    Transactivation of gene expression by Myc is inhibited by mutation at the phosphorylation sites Thr-58 and Ser-62. Gupta S, Seth A, Davis RJ. Proc Natl Acad Sci U S A. 1993 Apr 15;90(8):3216-20. PubMed 8386367