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pCB268
(Plasmid #53363)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 53363 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA3.0 - Nco
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5400
  • Total vector size (bp) 6964
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    DHFR
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Dhfr (a.k.a. 8430436I03Rik, AA607882, AI662710, AW555094)
  • Promoter CMV
  • Tag / Fusion Protein
    • Flag (N terminal on insert)

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site n/a (unknown if destroyed)
  • 5′ sequencing primer CMV-F
  • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    Ub
  • Species
    H. sapiens (human)
  • Tag / Fusion Protein
    • HA (N terminal on insert)

Cloning Information for Gene/Insert 2

Gene/Insert 3

  • Gene/Insert name
    Aβ42
  • Species
    H. sapiens (human)
  • Entrez Gene
    APP (a.k.a. AAA, ABETA, ABPP, AD1, APPI, CTFgamma, CVAP, PN-II, PN2, alpha-sAPP, preA4)
  • Tag / Fusion Protein
    • 13xMyc (C terminal on insert)

Cloning Information for Gene/Insert 3

  • Cloning method Restriction Enzyme
  • 5′ cloning site n/a (unknown if destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer n/a
  • 3′ sequencing primer BGH-rev
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
  • Articles Citing this Plasmid
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCB268 was a gift from Alexander Varshavsky (Addgene plasmid # 53363 ; http://n2t.net/addgene:53363 ; RRID:Addgene_53363)
  • For your References section:

    Neurodegeneration-associated protein fragments as short-lived substrates of the N-end rule pathway. Brower CS, Piatkov KI, Varshavsky A. Mol Cell. 2013 Apr 25;50(2):161-71. doi: 10.1016/j.molcel.2013.02.009. Epub 2013 Mar 14. 10.1016/j.molcel.2013.02.009 PubMed 23499006