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FW102 OL2-62
(Bacterial strain #53735)

No maps are available for this item.

Full plasmid sequence is not available for this item.


Item Catalog # Description Quantity Price (USD)
Bacterial Strain 53735 Bacteria in agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin and Streptomycin
  • Growth Temperature
  • Growth Strain(s)
  • Copy number


  • Gene/Insert name
  • Species

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

FW102 containing an F' Kan bearing the placOL2–62-lacZ fusion where the lambda-CI operator (OL2) is centered at position –62 relative to the start of transcription.
This is the reporter strain used with the Hochschild lab bacterial 2-hybrid system, as described in the following article:
This strain is also described in Deighan, P., Diez, C.M., Leibman, M., Hochschild, A., and Nickels, B.E. (2008) The bacteriophage λ Q
antiterminator protein contacts the β-flap domain of RNA polymerase, PNAS 105: 15305-15310

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    FW102 OL2-62 was a gift from Ann Hochschild (Addgene plasmid # 53735)
  • For your References section:

    Activation of prokaryotic transcription through arbitrary protein-protein contacts. Dove SL, Joung JK, Hochschild A. Nature. 1997 Apr 10;386(6625):627-30. 10.1038/386627a0 PubMed 9121589