FW102 OL2-62
(Bacterial strain #53735)

• Purpose: Bacterial-two hybrid reporter strain
• Depositing Lab: Ann Hochschild
• Publication: Dove et al Nature. 1997 Apr 10;386(6625):627-30.

Backbone

• Vector backbone: none

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin and Streptomycin
• Growth Temperature: 37°C
• Growth Strain(s): NA
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: none
• Species: E.coli

Resource Information

• Supplemental Documents:
  • Hochschild_B2H_image
  • Hochschild_B2H_info

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

FW102 containing an F' Kan bearing the placOL2–62-lacZ fusion where the lambda-CI operator (OL2) is centered at position –62 relative to the start of transcription.
This is the reporter strain used with the Hochschild lab bacterial 2-hybrid system, as described in the following article:
http://www.addgene.org/browse/article/8393/
This strain is also described in Deighan, P., Diez, C.M., Leibman, M., Hochschild, A., and Nickels, B.E. (2008) The bacteriophage λ Q
antiterminator protein contacts the β-flap domain of RNA polymerase, PNAS 105: 15305-15310