PurposeBacterial-two hybrid reporter strain
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Bacterial Strain||53735||Bacteria in agar stab||1||$75|
This material is available to academics and nonprofits only.
Growth in Bacteria
Bacterial Resistance(s)Kanamycin and Streptomycin
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
FW102 containing an F' Kan bearing the placOL2–62-lacZ fusion where the lambda-CI operator (OL2) is centered at position –62 relative to the start of transcription.
This is the reporter strain used with the Hochschild lab bacterial 2-hybrid system, as described in the following article:
This strain is also described in Deighan, P., Diez, C.M., Leibman, M., Hochschild, A., and Nickels, B.E. (2008) The bacteriophage λ Q
antiterminator protein contacts the β-flap domain of RNA polymerase, PNAS 105: 15305-15310
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:FW102 OL2-62 was a gift from Ann Hochschild (Addgene plasmid # 53735)
For your References section:Activation of prokaryotic transcription through arbitrary protein-protein contacts. Dove SL, Joung JK, Hochschild A. Nature. 1997 Apr 10;386(6625):627-30. 10.1038/386627a0 PubMed 9121589