Purposemammalian expression of flag-tagged ATR middle region
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||53768||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
Alt nameataxia telangiectasia and Rad3 related
SpeciesH. sapiens (human)
Mutationmiddle domain, aa 593-1700*
Entrez GeneATR (a.k.a. FCTCS, FRP1, MEC1, SCKL, SCKL1)
- Promoter CMV
/ Fusion Protein
- Flag (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGH Reverse (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
*R1631H polymorphism--not known to affect function
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pcDNA4-ATR-M was a gift from Aziz Sancar (Addgene plasmid # 53768 ; http://n2t.net/addgene:53768 ; RRID:Addgene_53768)
For your References section:Preferential binding of ATR protein to UV-damaged DNA. Unsal-Kacmaz K, Makhov AM, Griffith JD, Sancar A. Proc Natl Acad Sci U S A. 2002 May 14;99(10):6673-8. 10.1073/pnas.102167799 PubMed 12011431
Map uploaded by the depositor.