|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||54490||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerW. R. Jacobs
- Backbone size (bp) 4296
Modifications to backboneXbaI-NdeI insertion of hsp60 promoter, theophylline riboswitch, and SapI restriction site.
- Promoter hsp60
Growth in Bacteria
Growth instructionsShuttle vector: multi-copy episomal in E. coli, single-copy integrating in mycobacteria.
Copy numberLow Copy
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pRiboI SapNde was a gift from Jessica Seeliger (Addgene plasmid # 54490 ; http://n2t.net/addgene:54490 ; RRID:Addgene_54490)