pET StrepII-StrepII TEV LIC cloning vector (2RRT)
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||55215||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector typeBacterial Expression
/ Fusion Protein
- StrepII-StrepII (N terminal on backbone)
Growth in Bacteria
Growth Strain(s)XL1 Blue
Copy numberLow Copy
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer T7 Forward
- 3′ sequencing primer T7 reverse (Common Sequencing Primers)
This plasmid is an empty vector. Your gene can be inserted with a LIC cloning protocol. All 2-series vectors work as single-expression vectors, as well as transfer vectors for our polycistronic system.
The LIC cloning site is flanked by 5 pairs of restriction sites, so that your gene can easily be subcloned into our polycistronic destination vectors (2D, 2E, or 2Z).
2RRT has a TEV-cleavable N-terminal StrepII-StrepII tag.
To clone into this vector, add LIC v1 tags to the 5' end of your PCR primers.
Forward - 5'-TACTTCCAATCCAATGCA-3'
Reverse - 5'-TTATCCACTTCCAATGTTATTA-3'
Linearize the plasmid with SspI and gel purify.
When digesting the DNA with T4 polymerase, use dCTP for insert and dGTP for vector.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pET StrepII-StrepII TEV LIC cloning vector (2RRT) was a gift from Scott Gradia (Addgene plasmid # 55215 ; http://n2t.net/addgene:55215 ; RRID:Addgene_55215)