Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

Dyn1(K44A)-mRFP
(Plasmid #55795)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 55795 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    Derived from pEGFP-N1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 3950
  • Total vector size (bp) 7286
  • Modifications to backbone
    GFP was removed as a BamHI/NotI cassette and replaced with mRFP.
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH10B
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Dynamin-1(K44A)-mRFP
  • Alt name
    DNM1
  • Species
    R. norvegicus (rat); Discosoma sp.
  • Insert Size (bp)
    3336
  • Mutation
    Dynamin-1(K44A)-mRFP was produced from Dynamin-1(K44A)-GFP by replacing GFP with mRFP.
  • GenBank ID
  • Entrez Gene
    Dnm1 (a.k.a. Dnm)
  • Promoter CMV
  • Tag / Fusion Protein
    • mRFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site None (unknown if destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer CMV Forward
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Dyn1(K44A)-GFP was obtained from Pietro De Camilli, Howard Hughes Medical Institute, Yale University, New Haven, CT. mRFP was obtained from Roger Tsien, University of California, San Diego, California.
  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
  • Articles Citing this Plasmid
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Dyn1(K44A)-mRFP was a gift from Catherine Berlot (Addgene plasmid # 55795 ; http://n2t.net/addgene:55795 ; RRID:Addgene_55795)
  • For your References section:

    Live cell imaging of Gs and the beta2-adrenergic receptor demonstrates that both alphas and beta1gamma7 internalize upon stimulation and exhibit similar trafficking patterns that differ from that of the beta2-adrenergic receptor. Hynes TR, Mervine SM, Yost EA, Sabo JL, Berlot CH. J Biol Chem. 2004 Oct 15;279(42):44101-12. Epub 2004 Aug 5. 10.1074/jbc.M405151200 PubMed 15297467