Dyn1(K44A)-mRFP
(Plasmid #55795)

• Purpose: Dominant negative Dynamin 1 mutant tagged with mRFP
• Depositing Lab: Catherine Berlot
• Depositing Organization: Geisinger Health Center
  Located in United States of America
• Publication: Hynes et al J Biol Chem. 2004 Oct 15;279(42):44101-12. Epub 2004 Aug 5.
• Citations: 6 Articles

Backbone

• Vector backbone: Derived from pEGFP-N1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 3950
• Total vector size (bp): 7286
• Modifications to backbone: GFP was removed as a BamHI/NotI cassette and replaced with mRFP.
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH10B
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Dynamin-1(K44A)-mRFP
• Alt name: DNM1
• Species: R. norvegicus (rat); Discosoma sp.
• Insert Size (bp): 3336
• Mutation: Dynamin-1(K44A)-mRFP was produced from Dynamin-1(K44A)-GFP by replacing GFP with mRFP.
• GenBank ID:
• Entrez Gene: Dnm1 (a.k.a. Dnm)
• Promoter: CMV
• Tag / Fusion Protein:
  • mRFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: None (unknown if destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: CMV Forward

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Dyn1(K44A)-GFP was obtained from Pietro De Camilli, Howard Hughes Medical Institute, Yale University, New Haven, CT. mRFP was obtained from Roger Tsien, University of California, San Diego, California.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

DNA (Catalog # 55795-D50)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

• Amount: 50 μg
• Concentration: 1 μg/μL
• Pricing: $495 USD
• Storage: 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

DNA (Catalog # 55795-D100)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

• Amount: 100 μg
• Concentration: 1 μg/μL
• Pricing: $585 USD
• Storage: 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.