mPlum-VE-Cadherin-N-10
(Plasmid #56005)

• Purpose: Localization: Tight Junctions, Excitation: 590, Emission: 649
• Depositing Lab: Michael Davidson
• Publication: Davidson Red fluorescent proteins (unpublished)

Backbone

• Vector backbone: mPlum-N1
• Backbone size w/o insert (bp): 4750
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: VE-Cadherin
• Alt name: X79981.1
• Species: H. sapiens (human)
• Mutation: I503T
• Entrez Gene: CDH5 (a.k.a. 7B4, CD144)
• Promoter: CMV
• Tag / Fusion Protein:
  • mPlum (C terminal on backbone)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: SV40pA-R

Resource Information

• Supplemental Documents:
  • mPlum-VE-Cadherin-N-10 Data Supplement
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Excitation = 590; Emission = 649

Addgene QC NGS analysis identifies an I503T discrepancy in the insert sequence. It is unknown what, if any, impact this may have on plasmid function.