MC177VSG221RNAi
(Plasmid #59730)

• Purpose: For use in trypanosome cell lines already expressing T7 polymerase and Tet-repressor. Tetracycline induction results in the production of dsRNA targeting VSG221.
• Depositing Lab: Gloria Rudenko
• Publication: Sheader et al Proc Natl Acad Sci U S A. 2005 Jun 14;102(24):8716-21. Epub 2005 Jun 3.

Backbone

• Vector backbone: pBluescriptII
• Total vector size (bp): 2900
• Vector type: RNAi
• Selectable markers: Phleomycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: VSG221 RNAi fragment
• Species: T. brucei Lister 427
• Insert Size (bp): 803
• Promoter: opposing T7 promoters

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (unknown if destroyed)
• 3′ cloning site: XhoI (unknown if destroyed)
• 5′ sequencing primer: atacagaatgtctttggc

Resource Information

• Addgene Notes:
  • Addgene Diagnostic Digest

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that Addgene QC did not confirm the RNAi fragment, but the linear size of the digested vector give the predicted result.