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pSynSRE-T-Luc
(Plasmid #60444)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 60444 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 60444-D50 50 μg of DNA in Tris buffer $495
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DNA 60444-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pGL2 basic
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 5357
  • Total vector size (bp) 5504
  • Modifications to backbone
    Contains the hamster HMG-CoA synthase promoter sequence harboring SREBP responsive region (-324/-225) fused directly to minimal HMG-CoA sythase TATA box (-28/+39) that is linked to luciferase in polylinker of pGL2 basic
  • Vector type
    Mammalian Expression, Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HMG-CoA synthase promoter (-324/-225)
  • Alt name
    3-Hydroxy-3-methylglutaryl-coenzyme A synthase promoter
  • Species
    Hamster
  • Insert Size (bp)
    186
  • Promoter HMG-CoA synthase (-324/-225) & TATAA (-28/+39)
  • Tag / Fusion Protein
    • Luciferase (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SstI or SacI (not destroyed)
  • 3′ cloning site HindIII for promoter and TATAA; NheI for promoter region only (not destroyed)
  • 5′ sequencing primer GLprimer1
  • 3′ sequencing primer GLprimer2
  • (Common Sequencing Primers)

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSynSRE-T-Luc was a gift from Timothy Osborne (Addgene plasmid # 60444 ; http://n2t.net/addgene:60444 ; RRID:Addgene_60444)

  • For your References section:

    Sterol regulation of 3-hydroxy-3-methylglutaryl-coenzyme A synthase gene through a direct interaction between sterol regulatory element binding protein and the trimeric CCAAT-binding factor/nuclear factor Y. Dooley KA, Millinder S, Osborne TF. J Biol Chem. 1998 Jan 16;273(3):1349-56. PubMed 9430668
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