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pENTR-D-TOPO-PAmCherry1_1-159-MCS
(Plasmid #60545)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 60545 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pENTR/D-TOPO
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 2580
  • Total vector size (bp) 3090
  • Modifications to backbone
    Added MCS. C-terminal MCS was lost during cloning.
  • Vector type
    Cloning

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PAmCherry1 N-terminal fragment
  • Alt name
    RN1-159
  • Insert Size (bp)
    477
  • Mutation
    amino acids 1-159

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer M13 forward
  • 3′ sequencing primer M13 reverse
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

Parent plasmid (full-length PAmCherry1 in pENTR) (Addgene plasmid 60608) originally reported in Nan et al., 2013 (PMID:24158481).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pENTR-D-TOPO-PAmCherry1_1-159-MCS was a gift from Xiaolin Nan (Addgene plasmid # 60545 ; http://n2t.net/addgene:60545 ; RRID:Addgene_60545)
  • For your References section:

    Photoactivated localization microscopy with bimolecular fluorescence complementation (BiFC-PALM) for nanoscale imaging of protein-protein interactions in cells. Nickerson A, Huang T, Lin LJ, Nan X. PLoS One. 2014 Jun 25;9(6):e100589. doi: 10.1371/journal.pone.0100589. eCollection 2014. 10.1371/journal.pone.0100589 PubMed 24963703