Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

(Plasmid #60546)


Item Catalog # Description Quantity Price (USD)
Plasmid 60546 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 2580
  • Total vector size (bp) 2883
  • Modifications to backbone
    Added flanking MCSs
  • Vector type

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
    PAmCherry1 C-terminal fragment
  • Alt name
  • Insert Size (bp)
  • Mutation
    amino acids 160-236

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer M13 forward
  • 3′ sequencing primer M13 reverse
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Parent plasmid (full-length PAmCherry1 in pENTR) (Addgene plasmid 60608) originally reported in Nan et al., 2013 (PMID:24158481).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pENTR-D-TOPO-PAmCherry1_160-236-MCS was a gift from Xiaolin Nan (Addgene plasmid # 60546 ; ; RRID:Addgene_60546)
  • For your References section:

    Photoactivated localization microscopy with bimolecular fluorescence complementation (BiFC-PALM) for nanoscale imaging of protein-protein interactions in cells. Nickerson A, Huang T, Lin LJ, Nan X. PLoS One. 2014 Jun 25;9(6):e100589. doi: 10.1371/journal.pone.0100589. eCollection 2014. 10.1371/journal.pone.0100589 PubMed 24963703