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Purposemammalian expression vector
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Depositing Lab
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Depositing OrganizationSinai Health System
Located in Canada -
Citations
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 60813 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 * | |
| DNA | 60813-D50 | 50 μg of DNA in Tris buffer | $495 * | ||
| DNA | 60813-D100 | 100 μg of DNA in Tris buffer | $585 * | ||
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Backbone
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Vector backbonepcDNA5-FRT/T0-eGFP
- Backbone size w/o insert (bp) 5137
- Total vector size (bp) 11053
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Vector typeMammalian Expression
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Selectable markersHygromycin, Blasticidin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)XL1 Blue
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Copy numberUnknown
Gene/Insert
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Gene/Insert name53BP1
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SpeciesH. sapiens (human)
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Insert Size (bp)5916
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Entrez GeneTP53BP1 (a.k.a. 53BP1, TDRD30, p202, p53BP1)
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Tag
/ Fusion Protein
- eGFP (N terminal on backbone)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 60813-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 60813-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA5-FRT/TO-eGFP-53BP1 was a gift from Daniel Durocher (Addgene plasmid # 60813 ; http://n2t.net/addgene:60813 ; RRID:Addgene_60813) -
For your References section:
53BP1 is a reader of the DNA-damage-induced H2A Lys 15 ubiquitin mark. Fradet-Turcotte A, Canny MD, Escribano-Diaz C, Orthwein A, Leung CC, Huang H, Landry MC, Kitevski-LeBlanc J, Noordermeer SM, Sicheri F, Durocher D. Nature. 2013 Jul 4;499(7456):50-4. doi: 10.1038/nature12318. Epub 2013 Jun 12. 10.1038/nature12318 PubMed 23760478
Map uploaded by the depositor.
