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pTargeT gE
(Plasmid #60845)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 60845 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 60845-D50 50 μg of DNA in Tris buffer $495
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DNA 60845-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pTargeT
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 5700
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ORF68
  • Alt name
    VZV gE
  • Alt name
    varicella glycoprotein E
  • Insert Size (bp)
    1869
  • GenBank ID
    NC_001348.1
  • Entrez Gene
    ORF68 (a.k.a. HHV3_gp69)
  • Promoter CMV

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    N/A

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTargeT gE was a gift from Charles Grose (Addgene plasmid # 60845 ; http://n2t.net/addgene:60845 ; RRID:Addgene_60845)

  • For your References section:

    Autophagosome formation during varicella-zoster virus infection following endoplasmic reticulum stress and the unfolded protein response. Carpenter JE, Jackson W, Benetti L, Grose C. J Virol. 2011 Sep;85(18):9414-24. doi: 10.1128/JVI.00281-11. Epub 2011 Jul 13. 10.1128/JVI.00281-11 PubMed 21752906
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