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(Plasmid #60865)


Item Catalog # Description Quantity Price (USD)
Plasmid 60865 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 4304
  • Total vector size (bp) 6194
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    Low Copy

Gene/Insert 1

  • Gene/Insert name
  • Alt name
  • Species
    Synthetic; Bacillus stearothermophilus
  • Insert Size (bp)
  • Mutation
    Threonine 120 to Cysteine in Xylanase domain
  • Promoter T7
  • Tag / Fusion Protein
    • 6xHIS (N terminal on insert)

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site BspHI (destroyed during cloning)
  • 3′ cloning site KpnI (not destroyed)
  • 5′ sequencing primer T7 Forward
  • 3′ sequencing primer T7 Reverse
  • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
  • Alt name
    dockerin type III
  • Alt name
  • Species
    Ruminococcus flavefaciens
  • Insert Size (bp)
  • Promoter T7

Cloning Information for Gene/Insert 2

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer T7 Forward
  • 3′ sequencing primer T7 Reverse
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    We thankfully received the plasmid from Professor Edward Bayer (Department of Biological Chemistry, Weizmann Institute of Science, Israel) as recently described in this paper: "Atypical cohesin-dockerin complex responsible for cell surface attachment of cellulosomal components: binding fidelity, promiscuity, and structural buttresses."Salama-Alber Orly, Jobby Maroor K., Chitayat Seth, Smith Steven P., White Bryan A., Shimon Linda J. W., Lamed Raphael, Frolow Felix, Bayer Edward A. (The Journal of biological chemistry, 2013, 288 (23).

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET28a-HIS-Xyl-Xmod-DocIII was a gift from Hermann Gaub (Addgene plasmid # 60865 ; ; RRID:Addgene_60865)
  • For your References section:

    Ultrastable cellulosome-adhesion complex tightens under load. Schoeler C, Malinowska KH, Bernardi RC, Milles LF, Jobst MA, Durner E, Ott W, Fried DB, Bayer EA, Schulten K, Gaub HE, Nash MA. Nat Commun. 2014 Dec 8;5:5635. doi: 10.1038/ncomms6635. 10.1038/ncomms6635 PubMed 25482395