pSwith-tdTomato
(Plasmid
#60953)
-
PurposeCre recombinase-mediated cassette exchange
-
Depositing Lab
-
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 60953 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
-
Vector backbonepMXs
-
Backbone manufacturerGeneArt
- Backbone size w/o insert (bp) 2450
-
Vector typeCre/Lox
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nametdTomato
-
Insert Size (bp)1400
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (unknown if destroyed)
- 3′ cloning site SpeI (unknown if destroyed)
- 5′ sequencing primer M13-F20
- 3′ sequencing primer M13-R (Common Sequencing Primers)
Resource Information
-
Supplemental Documents
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pSwith-tdTomato was a gift from Liangxue Lai (Addgene plasmid # 60953 ; http://n2t.net/addgene:60953 ; RRID:Addgene_60953) -
For your References section:
Rosa26-targeted swine models for stable gene over-expression and Cre-mediated lineage tracing. Li X, Yang Y, Bu L, Guo X, Tang C, Song J, Fan N, Zhao B, Ouyang Z, Liu Z, Zhao Y, Yi X, Quan L, Liu S, Yang Z, Ouyang H, Chen YE, Wang Z, Lai L. Cell Res. 2014 Apr;24(4):501-4. doi: 10.1038/cr.2014.15. Epub 2014 Feb 7. 10.1038/cr.2014.15 PubMed 24503648
