pHT101-mCherry
(Plasmid #61021)

• Purpose: (Empty Backbone) Expresses mCherry tagged fusion proteins in worms
• Depositing Lab: Casonya Johnson
• Publication: Johnson lab C. elegans plasmids (unpublished)

Backbone

• Vector backbone: pPD95_67
• Backbone manufacturer: Andrew Fire (Addgene plasmid #1490)
• Backbone size (bp): 4526
• Modifications to backbone: mCherry was amplified from pCFJ90 (Addgene plasmid #19327) and inserted into pPD95_67 to replace GFP with mCherry.
• Vector type: Worm Expression
• Promoter: lac
• Tag / Fusion Protein:
  • mCherry

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: M13pUC-rev

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Constructed by Han-Ting Chou in the laboratory of Casonya Johnson.
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This vector can be used to create N- terminal or C-terminal mCherry tagged fusion proteins for expression in C. elegans. The mCherry and a modified MCS was inserted into the vector backbone using AgeI and EcoRI sites. See plasmid map and sequence for more information.

Note that mCherry has been codon optimized for C. elegans expression. See pCFJ90 (Plasmid #19327) for details on the mCherry protein sequence.