|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||61021||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Backbone manufacturerAndrew Fire (Addgene plasmid #1490)
- Backbone size (bp) 4526
Modifications to backbonemCherry was amplified from pCFJ90 (Addgene plasmid #19327) and inserted into pPD95_67 to replace GFP with mCherry.
Vector typeWorm Expression
- Promoter lac
/ Fusion Protein
Growth in Bacteria
Copy numberHigh Copy
- Cloning method Restriction Enzyme
- 5′ sequencing primer M13pUC-rev (Common Sequencing Primers)
This vector can be used to create N- terminal or C-terminal mCherry tagged fusion proteins for expression in C. elegans. The mCherry and a modified MCS was inserted into the vector backbone using AgeI and EcoRI sites. See plasmid map and sequence for more information.
Note that mCherry has been codon optimized for C. elegans expression. See pCFJ90 (Plasmid #19327) for details on the mCherry protein sequence.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pHT101-mCherry was a gift from Casonya Johnson (Addgene plasmid # 61021 ; http://n2t.net/addgene:61021 ; RRID:Addgene_61021)