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pHT101-mCherry
(Plasmid #61021)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 61021 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pPD95_67
  • Backbone manufacturer
    Andrew Fire (Addgene plasmid #1490)
  • Backbone size (bp) 4526
  • Modifications to backbone
    mCherry was amplified from pCFJ90 (Addgene plasmid #19327) and inserted into pPD95_67 to replace GFP with mCherry.
  • Vector type
    Worm Expression
  • Promoter lac
  • Tag / Fusion Protein
    • mCherry

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Cloning Information

Resource Information

Depositor Comments

This vector can be used to create N- terminal or C-terminal mCherry tagged fusion proteins for expression in C. elegans. The mCherry and a modified MCS was inserted into the vector backbone using AgeI and EcoRI sites. See plasmid map and sequence for more information.

Note that mCherry has been codon optimized for C. elegans expression. See pCFJ90 (Plasmid #19327) for details on the mCherry protein sequence.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHT101-mCherry was a gift from Casonya Johnson (Addgene plasmid # 61021 ; http://n2t.net/addgene:61021 ; RRID:Addgene_61021)