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PurposeGateway-Entry vector containing AtU6-26 promoter and sgRNA backbone
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 61432 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepGEM-T-easy
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Backbone manufacturerPromega Corp.
- Backbone size w/o insert (bp) 3000
- Total vector size (bp) 3700
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Vector typePlant Expression, CRISPR
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameAtU6-26:sgRNA
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gRNA/shRNA sequenceto be specified by cloning
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SpeciesA. thaliana (mustard weed), Synthetic
- Promoter AtU6-26
Cloning Information
- Cloning method TOPO Cloning
- 5′ sequencing primer M13fw
- 3′ sequencing primer M13rev (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEn-Chimera was a gift from Holger Puchta (Addgene plasmid # 61432 ; http://n2t.net/addgene:61432 ; RRID:Addgene_61432) -
For your References section:
Both CRISPR/Cas-based nucleases and nickases can be used efficiently for genome engineering in Arabidopsis thaliana. Fauser F, Schiml S, Puchta H. Plant J. 2014 Jul;79(2):348-59. doi: 10.1111/tpj.12554. Epub 2014 Jun 17. 10.1111/tpj.12554 PubMed 24836556
Map uploaded by the depositor.
