NET55 pEGFP-N2 (610)
Purposemammalian expression of nuclear envelope transmembrane protein
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||61992||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Vector typeMammalian Expression
Growth in Bacteria
Bacterial Resistance(s)Kanamycin, 50 μg/mL
Copy numberHigh Copy
SpeciesH. sapiens (human)
Entrez GeneAPH1B (a.k.a. APH-1B, PRO1328, PSFL, TAAV688)
- Promoter CMV
/ Fusion Protein
- EGFP (C terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site NheI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer mRFP1-R (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:NET55 pEGFP-N2 (610) was a gift from Eric Schirmer (Addgene plasmid # 61992 ; http://n2t.net/addgene:61992 ; RRID:Addgene_61992)
For your References section:System analysis shows distinct mechanisms and common principles of nuclear envelope protein dynamics. Zuleger N, Kelly DA, Richardson AC, Kerr AR, Goldberg MW, Goryachev AB, Schirmer EC. J Cell Biol. 2011 Apr 4;193(1):109-23. doi: 10.1083/jcb.201009068. Epub 2011 Mar 28. 10.1083/jcb.201009068 PubMed 21444689