pGTy1mhis3-AI or pGTy1his3-AI
(Plasmid #62228)

• Purpose: A Ty1 element tagged with the retrotransposition indicator gene his3-AI is fused to the GAL1 promoter on a multicopy URA3-based shuttle plasmid.
• Depositing Lab: David Garfinkel
• Publication: Curcio et al Proc Natl Acad Sci U S A. 1991 Feb 1;88(3):936-40.

Backbone

• Vector backbone: unknown, pCGS42
• Backbone size w/o insert (bp): 5500
• Vector type: Yeast Expression
• Selectable markers: URA3, HIS3

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Ty1H3 retrotransposon
• Species: S. cerevisiae (budding yeast)
• Insert Size (bp): 8000
• GenBank ID: M18706.1
• Promoter: GAL1

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: ClaI (not destroyed)
• 3′ cloning site: ClaI (not destroyed)
• 5′ sequencing primer: obtain from GAL1 sequence
• 3′ sequencing primer: obtain from pBR322 sequence

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Industry MTA