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(Plasmid #62237)


Item Catalog # Description Quantity Price (USD)
Plasmid 62237 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


  • Vector backbone
    clontech N1
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 4000
  • Total vector size (bp) 4900
  • Vector type
    Mammalian Expression
  • Selectable markers

Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
  • Species
    Synthetic; rabbit
  • Insert Size (bp)
  • Mutation
    signal anchor of rabbit cytochrome p450 and monomerized EGFP
  • Promoter CMV
  • Tag / Fusion Protein
    • mGFP

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer tggtttagtgaaccgt
  • 3′ sequencing primer GTTCAGGGGGAGGTGTG
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

Used for assessing the likelihood of a fluorescent protein to oligomerize in cells. This is the monomeric control protein that does NOT form Organized Smooth Endoplasmic Reticulum (OSER) structures.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CyTERM-mGFP was a gift from Erik Snapp (Addgene plasmid # 62237 ; ; RRID:Addgene_62237)
  • For your References section:

    Assessing the tendency of fluorescent proteins to oligomerize under physiologic conditions. Costantini LM, Fossati M, Francolini M, Snapp EL. Traffic. 2012 May;13(5):643-9. doi: 10.1111/j.1600-0854.2012.01336.x. Epub 2012 Feb 20. 10.1111/j.1600-0854.2012.01336.x PubMed 22289035