Purposeused to characterize the promoter activities of PTet for dCas9 and sgRNA response functions.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||62245||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Total vector size (bp) 8285
Vector typeBacterial Expression
Growth in Bacteria
- Promoter Ptet
- Cloning method Unknown
- 5′ sequencing primer kan-R
- 3′ sequencing primer LacI-R (Common Sequencing Primers)
Terms and Licenses
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pAN-PTet-YFP was a gift from Christopher Voigt (Addgene plasmid # 62245 ; http://n2t.net/addgene:62245 ; RRID:Addgene_62245)
For your References section:Multi-input CRISPR/Cas genetic circuits that interface host regulatory networks. Nielsen AA, Voigt CA. Mol Syst Biol. 2014 Nov 24;10:763. doi: 10.15252/msb.20145735. PubMed 25422271