pET-NLS-Cas9-6xHis Citations (18)
Originally described in: Cationic lipid-mediated delivery of proteins enables efficient protein-based genome editing in vitro and in vivo.Zuris JA, Thompson DB, Shu Y, Guilinger JP, Bessen JL, Hu JH, Maeder ML, Joung JK, Chen ZY, Liu DR Nat Biotechnol. 2015 Jan;33(1):73-80. doi: 10.1038/nbt.3081. Epub 2014 Oct 30. PubMed Journal
Articles Citing pET-NLS-Cas9-6xHis
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| Poly-sgRNA/siRNA ribonucleoprotein nanoparticles for targeted gene disruption. Ha JS, Lee JS, Jeong J, Kim H, Byun J, Kim SA, Lee HJ, Chung HS, Lee JB, Ahn DR. J Control Release. 2017 Mar 28;250:27-35. doi: 10.1016/j.jconrel.2017.02.007. Epub 2017 Feb 4. PubMed |
| Incorporation of bridged nucleic acids into CRISPR RNAs improves Cas9 endonuclease specificity. Cromwell CR, Sung K, Park J, Krysler AR, Jovel J, Kim SK, Hubbard BP. Nat Commun. 2018 Apr 13;9(1):1448. doi: 10.1038/s41467-018-03927-0. PubMed |
| Dynamics of PARKIN-Dependent Mitochondrial Ubiquitylation in Induced Neurons and Model Systems Revealed by Digital Snapshot Proteomics. Ordureau A, Paulo JA, Zhang W, Ahfeldt T, Zhang J, Cohn EF, Hou Z, Heo JM, Rubin LL, Sidhu SS, Gygi SP, Harper JW. Mol Cell. 2018 Apr 19;70(2):211-227.e8. doi: 10.1016/j.molcel.2018.03.012. Epub 2018 Apr 12. PubMed |
| Versatile High-Throughput Fluorescence Assay for Monitoring Cas9 Activity. Seamon KJ, Light YK, Saada EA, Schoeniger JS, Harmon B. Anal Chem. 2018 Jun 5;90(11):6913-6921. doi: 10.1021/acs.analchem.8b01155. Epub 2018 May 24. PubMed |
| HPV Oncogene Manipulation Using Nonvirally Delivered CRISPR/Cas9 or Natronobacterium gregoryi Argonaute. Lao YH, Li M, Gao MA, Shao D, Chi CW, Huang D, Chakraborty S, Ho TC, Jiang W, Wang HX, Wang S, Leong KW. Adv Sci (Weinh). 2018 May 18;5(7):1700540. doi: 10.1002/advs.201700540. eCollection 2018 Jul. PubMed |
| Optimization of sand fly embryo microinjection for gene editing by CRISPR/Cas9. Martin-Martin I, Aryan A, Meneses C, Adelman ZN, Calvo E. PLoS Negl Trop Dis. 2018 Sep 4;12(9):e0006769. doi: 10.1371/journal.pntd.0006769. eCollection 2018 Sep. PubMed |
| High-throughput targeted gene deletion in the model mushroom Schizophyllum commune using pre-assembled Cas9 ribonucleoproteins. Jan Vonk P, Escobar N, Wosten HAB, Lugones LG, Ohm RA. Sci Rep. 2019 May 21;9(1):7632. doi: 10.1038/s41598-019-44133-2. PubMed |
| A Two-Step Method for Obtaining Highly Pure Cas9 Nuclease for Genome Editing, Biophysical, and Structural Studies. Rajagopalan N, Kagale S, Bhowmik P, Song H. Methods Protoc. 2018 May 30;1(2). pii: mps1020017. doi: 10.3390/mps1020017. PubMed |
| Gold Nanocluster-Mediated Efficient Delivery of Cas9 Protein through pH-Induced Assembly-Disassembly for Inactivation of Virus Oncogenes. Ju E, Li T, Ramos da Silva S, Gao SJ. ACS Appl Mater Interfaces. 2019 Sep 25;11(38):34717-34724. doi: 10.1021/acsami.9b12335. Epub 2019 Sep 10. PubMed |
| Global Landscape and Dynamics of Parkin and USP30-Dependent Ubiquitylomes in iNeurons during Mitophagic Signaling. Ordureau A, Paulo JA, Zhang J, An H, Swatek KN, Cannon JR, Wan Q, Komander D, Harper JW. Mol Cell. 2020 Mar 5;77(5):1124-1142.e10. doi: 10.1016/j.molcel.2019.11.013. PubMed |
| Loss of splicing factor IK impairs normal skeletal muscle development. Ka HI, Seo H, Choi Y, Kim J, Cho M, Choi SY, Park S, Han S, An J, Chung HS, Yang Y, Kim MJ. BMC Biol. 2021 Apr 1;19(1):44. doi: 10.1186/s12915-021-00980-y. PubMed |
| Reversible switching of primary cells between normal and malignant state by oncogenic virus KSHV and CRISPR/Cas9-mediated targeting of a major viral latent protein. Ju E, Li T, Ramos da Silva S, Markazi A, Gao SJ. J Med Virol. 2021 Aug;93(8):5065-5075. doi: 10.1002/jmv.27046. Epub 2021 May 3. PubMed |
Evolution of CRISPR-associated endonucleases as inferred from resurrected proteins.
Alonso-Lerma B, Jabalera Y, Samperio S, Morin M, Fernandez A, Hille LT, Silverstein RA, Quesada-Ganuza A, Reifs A, Fernandez-Penalver S, Benitez Y, Soletto L, Gavira JA, Diaz A, Vranken W, Sanchez-Mejias A, Guell M, Mojica FJM, Kleinstiver BP, Moreno-Pelayo MA, Montoliu L, Perez-Jimenez R.
Nat Microbiol. 2023 Jan;8(1):77-90. doi: 10.1038/s41564-022-01265-y. Epub 2023 Jan 2.
PubMed
Associated Plasmids
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| Anti-SpCas9 IgY Polyclonal Antibodies Production for CRISPR Research Use. Leon E, Ortiz V, Perez A, Tellez J, Diaz GJ, Ramirez H MH, Contreras R LE. ACS Omega. 2023 Sep 6;8(37):33809-33818. doi: 10.1021/acsomega.3c04273. eCollection 2023 Sep 19. PubMed |
| Global cellular proteo-lipidomic profiling of diverse lysosomal storage disease mutants using nMOST. Kraus F, He Y, Swarup S, Overmyer KA, Jiang Y, Brenner J, Capitanio C, Bieber A, Jen A, Nightingale NM, Anderson BJ, Lee C, Paulo JA, Smith IR, Plitzko JM, Gygi SP, Schulman BA, Wilfling F, Coon JJ, Harper JW. Sci Adv. 2025 Jan 24;11(4):eadu5787. doi: 10.1126/sciadv.adu5787. Epub 2025 Jan 22. PubMed |
Cas9-mediated gene-editing frequency in microalgae is doubled by harnessing the interaction between importin alpha and phytopathogenic NLSs.
Le TT, Choi HI, Kim JW, Yun JH, Lee YH, Jeon EJ, Kwon KK, Cho DH, Choi DY, Park SB, Yoon HR, Lee J, Sim EJ, Lee YJ, Kim HS.
Proc Natl Acad Sci U S A. 2025 Mar 11;122(10):e2415072122. doi: 10.1073/pnas.2415072122. Epub 2025 Mar 3.
PubMed
Associated Plasmids |
| Phototropin connects blue light perception to starch metabolism in green algae. Yuan Y, Iannetta AA, Kim M, Sadecki PW, Arend M, Tsichla A, Aguila Ruiz-Sola M, Kepesidis G, Falconet D, Thevenon E, Tardif M, Brugiere S, Coute Y, Kleman JP, Sizova I, Schilling M, Jouhet J, Hegemann P, Li-Beisson Y, Nikoloski Z, Bastien O, Hicks LM, Petroutsos D. Nat Commun. 2025 Mar 15;16(1):2545. doi: 10.1038/s41467-025-57809-3. PubMed |
| A galactose-based auto-expression system improves T7-inducible protein production in Escherichia coli. Bosco J, Gagliano E, Boshae KL, Statz JP, Wheeler TB, Cuello D, Sliter A, Newby C, Lin B, Demeler A, Pierpont CL, Yates-Hansen C, Sydor MJ, Ferrini ME, Kuch KC, Cooper BS, Piggott BJ, Certel SJ, Hansen KB, Sprang SR, Bowler B, McClelland L, Berkmen M, Voronina E. Sci Rep. 2025 Mar 15;15(1):8936. doi: 10.1038/s41598-025-91954-5. PubMed |
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