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pGS1011
(Plasmid #63783)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 63783 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pUC57
  • Backbone manufacturer
    Fermentas/Thermo
  • Backbone size w/o insert (bp) 2710
  • Total vector size (bp) 3724
  • Vector type
    E. coli cloning vector
  • Selectable markers
    TRP1

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Strep-tag II followed by TRP1 selection marker
  • Species
    Synthetic
  • Promoter none
  • Tag / Fusion Protein
    • Strep-tag II (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site PaeI/SphI (not destroyed)
  • 5′ sequencing primer M13 uni -43
  • 3′ sequencing primer M13 rev -49
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry

Depositor Comments

The Strep-tag II sequence is adapted from the sequence of commercial Strep-tag II plasmids offered by IBA, Goettingen, Germany.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGS1011 was a gift from Monika Golas & Bjoern Sander (Addgene plasmid # 63783 ; http://n2t.net/addgene:63783 ; RRID:Addgene_63783)
  • For your References section:

    Strep-tag II and Twin-Strep Based Cassettes for Protein Tagging by Homologous Recombination and Characterization of Endogenous Macromolecular Assemblies in Saccharomyces cerevisiae. Rai J, Pemmasani JK, Voronovsky A, Jensen IS, Manavalan A, Nyengaard JR, Golas MM, Sander B. Mol Biotechnol. 2014 Jun 27. 10.1007/s12033-014-9778-5 PubMed 24969434