pBad_crtEBI_27
(Plasmid
#63806)
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Purposeexpresses crtEBI genes for neurosporene production
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 63806 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backboneFTV
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature30°C
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Growth Strain(s)DH10B
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namecrtEBI operon
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SpeciesRhodobactor
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MutationCodon optimized for E.coli
- Promoter PBAD
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer pBAD-F
- 3′ sequencing primer rrnB-T1-term-F (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Needs to be grown in E_IF3_15 strain for overproduction of neurosporene
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBad_crtEBI_27 was a gift from Howard Salis (Addgene plasmid # 63806 ; http://n2t.net/addgene:63806 ; RRID:Addgene_63806) -
For your References section:
Efficient search, mapping, and optimization of multi-protein genetic systems in diverse bacteria. Farasat I, Kushwaha M, Collens J, Easterbrook M, Guido M, Salis HM. Mol Syst Biol. 2014 Jun 21;10:731. doi: 10.15252/msb.20134955. PubMed 24952589
