Luc-Hdm4-3'UTR-F2d5
(Plasmid #64018)

• Purpose: Luciferase reporter of human Hdm4 3'UTR F2 fragment mutant #5
• Depositing Lab: Lin He
• Publication: Okada et al Genes Dev. 2014 Mar 1;28(5):438-50. doi: 10.1101/gad.233585.113. Epub 2014 Feb 14.

Backbone

• Vector backbone: pGL3-Control
• Backbone manufacturer: Promega
• Backbone size w/o insert (bp): 5256
• Total vector size (bp): 7447
• Modifications to backbone: att L1 and L2 cassette were first cloned to XbaI site for Gateway cloning
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Top10
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: HDMX
• Alt name: MDM4; MDMX; MRP1
• Species: H. sapiens (human)
• Insert Size (bp): 1795
• Entrez Gene: MDM4 (a.k.a. BMFS6, HDMX, MDMX, MRP1)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: GLprimer2
• 3′ sequencing primer: RVprimer4

Resource Information

• Supplemental Documents:
  • Luc-Hdm4-3'UTR-F2d5

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

HDM4 3'UTR F2 fragment that one (#5) of three miR-34a target sites was deleted