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Luc-Hdm4-3'UTR-F2d5
(Plasmid #64018)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 64018 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pGL3-Control
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 5256
  • Total vector size (bp) 7447
  • Modifications to backbone
    att L1 and L2 cassette were first cloned to XbaI site for Gateway cloning
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HDMX
  • Alt name
    MDM4; MDMX; MRP1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1795
  • Entrez Gene
    MDM4 (a.k.a. BMFS6, HDMX, MDMX, MRP1)

Cloning Information

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

HDM4 3'UTR F2 fragment that one (#5) of three miR-34a target sites was deleted

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Luc-Hdm4-3'UTR-F2d5 was a gift from Lin He (Addgene plasmid # 64018 ; http://n2t.net/addgene:64018 ; RRID:Addgene_64018)
  • For your References section:

    A positive feedback between p53 and miR-34 miRNAs mediates tumor suppression. Okada N, Lin CP, Ribeiro MC, Biton A, Lai G, He X, Bu P, Vogel H, Jablons DM, Keller AC, Wilkinson JE, He B, Speed TP, He L. Genes Dev. 2014 Mar 1;28(5):438-50. doi: 10.1101/gad.233585.113. Epub 2014 Feb 14. 10.1101/gad.233585.113 PubMed 24532687