GFP-BICD2N
(Plasmid #64070)

• Purpose: N-terminal 400 amino acids of mouse BICD2 codon optimized for Sf9 expression with a N-terminal GFP tag and a TEV-cleavable His-ZZ tag for purification (GFP-BICD2N)
• Depositing Lab: Andrew Carter
• Publication: Schlager et al EMBO J. 2014 Sep 1;33(17):1855-68. doi: 10.15252/embj.201488792. Epub 2014 Jul 1.

Backbone

• Vector backbone: pOmniBac
• Backbone size w/o insert (bp): 5000
• Total vector size (bp): 6960
• Vector type: Insect Expression

Growth in Bacteria

• Bacterial Resistance(s): Gentamicin, 10 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Top10
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: BICD2N (1-400)
• Species: M. musculus (mouse)
• Insert Size (bp): 1200
• Mutation: Codon optimized for Sf9 expression
• GenBank ID: NM_029791.4
• Entrez Gene: Bicd2
• Tags / Fusion Proteins:
  • His-ZZ-TCS (N terminal on backbone)
  • GFP (N terminal on backbone)

Resource Information

• Supplemental Documents:
  • GFP-BICD2N GenBank
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry