Coho salmon GLUT4 (okGLUT4)
(Plasmid
#65102)
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PurposeFull length SLC2A4 cDNA (Oncorhynchus kisutch)
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 65102 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepBluescript SK-
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Backbone manufacturerStratagene
- Backbone size w/o insert (bp) 3000
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSLC2A4
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Alt nameGLUT4 (Glucose transporter 4)
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Speciescoho salmon (Oncorhynchus kisutch)
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Insert Size (bp)3100
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GenBank IDAF502957
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer T3
- 3′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Coho salmon GLUT4 (okGLUT4) was a gift from Josep Planas (Addgene plasmid # 65102 ; http://n2t.net/addgene:65102 ; RRID:Addgene_65102) -
For your References section:
Functional characterization of an insulin-responsive glucose transporter (GLUT4) from fish adipose tissue. Capilla E, Diaz M, Albalat A, Navarro I, Pessin JE, Keller K, Planas JV. Am J Physiol Endocrinol Metab. 2004 Aug;287(2):E348-57. Epub 2004 Apr 27. 10.1152/ajpendo.00538.2003 PubMed 15113704