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NG1237 pCI-TPI-PTC160-4H
(Plasmid #65804)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 65804 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pCI-neo
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 4572
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    TPI
  • Alt name
    triosephosphate isomerase 1
  • Species
    H. sapiens (human)
  • Mutation
    premature stop codon at 160
  • Entrez Gene
    TPI1 (a.k.a. HEL-S-49, TIM, TPI, TPID)
  • Promoter CMV
  • Tag / Fusion Protein
    • 4H (4 copies of short beta-globin 3'UTR for probe binding) (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    NG1237 pCI-TPI-PTC160-4H was a gift from Niels Gehring (Addgene plasmid # 65804 ; http://n2t.net/addgene:65804 ; RRID:Addgene_65804)
  • For your References section:

    3' UTR length and messenger ribonucleoprotein composition determine endocleavage efficiencies at termination codons. Boehm V, Haberman N, Ottens F, Ule J, Gehring NH. Cell Rep. 2014 Oct 23;9(2):555-68. doi: 10.1016/j.celrep.2014.09.012. Epub 2014 Oct 9. 10.1016/j.celrep.2014.09.012 PubMed 25310981