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pOPINK-TRABID (NZF1-3, aa 1-178)
(Plasmid #66185)

Full plasmid sequence is not available for this item.



Item Catalog # Description Quantity Price (USD)
Plasmid 66185 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 6299
  • Total vector size (bp) 6833
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
  • Species
    H. sapiens (human)
  • Insert Size (bp)
  • Mutation
    only codes for amino acids 1-178 of TRABID
  • GenBank ID
  • Entrez Gene
    ZRANB1 (a.k.a. TRABID)
  • Promoter T7
  • Tag / Fusion Protein
    • His6-GST (N terminal on backbone)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pOPINK-TRABID (NZF1-3, aa 1-178) was a gift from David Komander (Addgene plasmid # 66185 ; ; RRID:Addgene_66185)
  • For your References section:

    Assembly and specific recognition of k29- and k33-linked polyubiquitin. Michel MA, Elliott PR, Swatek KN, Simicek M, Pruneda JN, Wagstaff JL, Freund SM, Komander D. Mol Cell. 2015 Apr 2;58(1):95-109. doi: 10.1016/j.molcel.2015.01.042. Epub 2015 Mar 5. 10.1016/j.molcel.2015.01.042 PubMed 25752577