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pAAV Syn hBE Rh Guanylyl Cyclase1 2A tDimer
(Plasmid #66779)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 66779 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pAAV
  • Backbone manufacturer
    Stratagene
  • Backbone size w/o insert (bp) 4562
  • Total vector size (bp) 7964
  • Vector type
    Mammalian Expression, AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL1 Blue
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    hbeRhGC
  • Alt name
    humanized Rhodopsin Guanylyl Cyclase 1
  • Alt name
    fungal rhodopsin guanylyl cyclase
  • Species
    Blastocladiella emersonii
  • Insert Size (bp)
    1878
  • GenBank ID
    KP731361 KF309499
  • Promoter human Synapsin

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site KpnI (not destroyed)
  • 5′ sequencing primer GACTCAGCGCTGCCTCAGTCTG;CTGCGGAGTAGAAAAAGC;GCCAGAAACCCAGAGAAGA;GCGGCCTGAGGTGGATTCG;GACGCTTATCTGGGAGTG
  • 3′ sequencing primer CGCTCCCACTTGAAGCCCTC
  • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    red fluorescent protein
  • Alt name
    tDimer 2
  • Species
    Discoma sp.
  • Insert Size (bp)
    1395
  • Promoter human Synapsin

Cloning Information for Gene/Insert 2

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer ggcagaggaagtcttctaacat
  • 3′ sequencing primer gtaatccagaggttgattatcg
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    hBE Rh GC originally from P.Hegemann-HU-Berlin Germany tDimer originally from R.Tsien USD USA
  • Terms and Licenses
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV Syn hBE Rh Guanylyl Cyclase1 2A tDimer was a gift from Thomas Oertner (Addgene plasmid # 66779)
  • For your References section:

    The rhodopsin-guanylyl cyclase of the aquatic fungus Blastocladiella emersonii enables fast optical control of cGMP signaling. Scheib U, Stehfest K, Gee CE, Korschen HG, Fudim R, Oertner TG, Hegemann P. Sci Signal. 2015 Aug 11;8(389):rs8. doi: 10.1126/scisignal.aab0611. 10.1126/scisignal.aab0611 PubMed 26268609