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(Plasmid #66798)


Item Catalog # Description Quantity Price (USD)
Plasmid 66798 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
    John Olsen UNC
  • Backbone size w/o insert (bp) 4876
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
    Floxed Chr2-YFP
  • Species
    Chlamydomonas reinhardtii
  • Insert Size (bp)
  • Mutation
  • Promoter CMVTetO7 Promoter
  • Tag / Fusion Protein
    • citrine (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site NheI (unknown if destroyed)
  • 5′ sequencing primer WPRE-R
  • 3′ sequencing primer EGFP-N
  • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    John Olsen, UNC

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEIAV-DIO-TLoop-CHR2YFP was a gift from Edward Callaway (Addgene plasmid # 66798 ; ; RRID:Addgene_66798)
  • For your References section:

    Optical control of retrogradely infected neurons using drug-regulated "TLoop" lentiviral vectors. Cetin A, Callaway EM. J Neurophysiol. 2014 May;111(10):2150-9. doi: 10.1152/jn.00495.2013. Epub 2014 Feb 26. 10.1152/jn.00495.2013 PubMed 24572099