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pTER RB1shRNA human
(Plasmid #66886)


Full plasmid sequence is not available for this item.


Item Catalog # Description Quantity Price (USD)
Plasmid 66886 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
    A gift from Dr. Marc van de Wetering (PMID: 12776180)
  • Vector type
    Mammalian Expression, RNAi
  • Selectable markers

Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
    retinoblastoma 1
  • gRNA/shRNA sequence
  • Species
    H. sapiens (human)
  • Entrez Gene
    RB1 (a.k.a. OSRC, PPP1R130, RB, p105-Rb, p110-RB1, pRb, pp110)
  • Promoter H1 Tet

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer pQE promoter
  • 3′ sequencing primer BGH-rev
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTER RB1shRNA human was a gift from Maria Alvarado-Kristensson (Addgene plasmid # 66886 ; ; RRID:Addgene_66886)
  • For your References section:

    Tumors with nonfunctional retinoblastoma protein are killed by reduced gamma-tubulin levels. Ehlen A, Rossello CA, von Stedingk K, Hoog G, Nilsson E, Pettersson HM, Jirstrom K, Alvarado-Kristensson M. J Biol Chem. 2012 May 18;287(21):17241-7. doi: 10.1074/jbc.M112.357038. Epub 2012 Apr 6. 10.1074/jbc.M112.357038 PubMed 22493456