Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more


(Plasmid #66896)


Item Catalog # Description Quantity Price (USD)
Plasmid 66896 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
    pMK-RQ (kanR)
  • Backbone manufacturer
    GeneArt (LifeTechnologies)
  • Backbone size w/o insert (bp) 2278
  • Total vector size (bp) 2679

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number


  • Gene/Insert name
  • Alt name
    Leader Of Cox8 Repeated
  • Alt name
    Synthetic variants of COX8A MTS
  • Species
  • Insert Size (bp)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SfiI (not destroyed)
  • 3′ cloning site SfiI (not destroyed)
  • 5′ sequencing primer n/a
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The LOC8R targeting sequence was designed by Benjamin S. Padman during his doctoral research project (Primary supervisor: Georg Ramm)

Insert into an N1 vector by subcloning. The number of Cox8 MTS repeats in LOC8R can be modified as follows:
4x Cox8: Insert at HindIII & AgeI sites. (subclone directly).
3x Cox8: Digest with BamHI and re-ligate. insert at HindIII & AgeI sites.
2x Cox8: Digest with XhoI and re-ligate. insert at HindIII & AgeI sites.
1x Cox8: Digest with HindIII and BamHI. Insert at HindIII & BamHI sites.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    N1-LOC8R was a gift from Georg Ramm (Addgene plasmid # 66896 ; ; RRID:Addgene_66896)