PurposeLeader Of Cox8 Repeated (LOC8R) is a synthetic mitochondrial targeting sequence compatible with any N1 vector. Internal restriction sites enable modular subcloning of 1x, 2x, 3x or 4x presequences.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||66896||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Vector backbonepMK-RQ (kanR)
Backbone manufacturerGeneArt (LifeTechnologies)
- Backbone size w/o insert (bp) 2278
- Total vector size (bp) 2679
Growth in Bacteria
Bacterial Resistance(s)Kanamycin, 50 μg/mL
Alt nameLeader Of Cox8 Repeated
Alt nameSynthetic variants of COX8A MTS
Insert Size (bp)401
- Cloning method Restriction Enzyme
- 5′ cloning site SfiI (not destroyed)
- 3′ cloning site SfiI (not destroyed)
- 5′ sequencing primer n/a (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
The LOC8R targeting sequence was designed by Benjamin S. Padman during his doctoral research project (Primary supervisor: Georg Ramm)
Insert into an N1 vector by subcloning. The number of Cox8 MTS repeats in LOC8R can be modified as follows:
4x Cox8: Insert at HindIII & AgeI sites. (subclone directly).
3x Cox8: Digest with BamHI and re-ligate. insert at HindIII & AgeI sites.
2x Cox8: Digest with XhoI and re-ligate. insert at HindIII & AgeI sites.
1x Cox8: Digest with HindIII and BamHI. Insert at HindIII & BamHI sites.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:N1-LOC8R was a gift from Georg Ramm (Addgene plasmid # 66896 ; http://n2t.net/addgene:66896 ; RRID:Addgene_66896)